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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034 M.Sc.DEGREE EXAMINATION BIOTECHNOLOGY THIRDSEMESTER APRIL 2017
BT 3956- FUNCTIONAL GENOMICS
Date: 24-04-2017 Dept. No. Max. 100 Marks
09:00-12:00 PART A Answer ALL the Questions I. Choose the correct answer x 1 5 Marks)
1. Which of the following has largest genome?
a. Human b. E.coli c. Pisum sativum d. Amoeba dubia
2. Which method is used for quantification of RNA?
Microarray RT-qPCR Mass Spectrometry Northern Blotting
3. the proteomic approach to determine how proteins interact with each other in living systems.
a)Mining b)Profiling c)Protein network mapping d)None of the above
4. Which of the following uses antibodies to study proteins?
SPR FRET Mass Spectrometry Pull down assay
5. Which among the following technique was used to study β globin gene?
RNAi Gene knockout Chemical mutagenesis Insertional mutagenesis
II. State whether the following are true or false (5x1=5 Marks)
6. Esterase is used to degrade the excess nucleotide bases
7. Operons are not found in eucaryotes
8. MPSS is a tag based technology.
9. RNAi is a post transcriptional process.
10. snRNAs are small nucleolar RNAs.
III. Complete the following x 5 Marks)
11. The number of genes in yeast is
12. The company that introduced photolithograhy for making arrays
13. Cy 5 gives fluorescence
14. In MS, the greater the m/z ration, the the speed of the ions
15. Long ds RNA are cleaved by
IV. Answer the following, each within 50 words x 1 5 Marks)
16. What is C value paradox?
17. Define metabolic profiling?
18. Mention a tag based technology for transcriptomics
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19. State any one advantage of dye flip in microarray.
20. What is pharmacodynamics?
PART B Answer the following, each within 500 words. Draw diagrams wherever necessary. x 8 40 Marks)
21. Comment on Yeast and E.coli as model organisms
OR (b)Discuss the complexities of eucaryotic genome.
22. Employ a technique to estimate DNA during PCR.
OR (b)Compare SAGE and MPSS.
23. Employ a technique to estimate DNA during PCR.
OR Differentiate between cDNA array and oilgonucleotide array.
24. Elucidate with examples how online resources have enhanced the study of protein-protein
interactions. OR (b)Review about the different types of protein- protein interactions.
25. (a)Briefly explain gene knockout through homologous recombination.
. OR Outline the steps involved in metagenomics. PART C Answer any TWO of the following, each within 1500 words. Draw diagrams wherever necessary. 2 x 20 40 Marks)
26. Write in detail about gene expression study using microarray technology.
27. Elaborate on pyrosequencing and ion torrent sequencing.
28. Describe in detail any four experimental techniques to study protein-protein interaction
29. Explain in detail about any two reverse genetics approach.