M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Biotechnology
Core XVI — PLANT BIOTECHNOLOGY — LAB VI
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. What are the agents used for the solidification of
medium?
2. How to adjust the pH of the medium?
3. Somatic embryo.
4. ISSR analysis.
5. Encapsulation.
6. What is the temperature recommended for
cryopreservation?
7. What is the role of phenol in DNA extraction?
8. Triparental mating.
9. What is term selection means?
10. What is the substrate used for GUS assay?
Sub. Code
501305
RW-841
2
WSS
Part B x 5 25)
Answer all questions, choosing either or
11. Explain the apical meristem explant preparation
and give the advantages of using meristem as
explant.
Or
What is filter sterilization? Write its advantages
over autoclaving.
12. Write the acclimatization procedures of
micropropagated plant.
Or
Draw the Dicotyledon somatic embryo stages and
explain.
13. Give the methodology of micropropagation of
endangered medicinal plants.
Or
Write the different facilities available for
cryopreservation.
14. How the purity of plant genomic DNA is confirmed?
Or
Give the methodology for the isolation of Ti plasmid.
15. Explain the procedure for GUS assay.
Or
Explain the Agrobacterium culture initiation,
infection and cocultivation process.
RW-841
3
WSS
Part C x 10 30)
Answer any three questions.
16. Explain the stock solution preparation for plant tissue
culture medium.
17. How suspension cultures are established and maintained?
18. Give the procedures for making synthetic seeds.
19. Discuss the methodology for the introduction of plasmids
into Agrobacterium cells.
20. Explain the principle and methodology for Biolistic
transformation.
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