M.Sc. DEGREE EXAMINATION, NOVEMBER 2017.
Third Semester
Biotechnology
Core XV LAB V RECOMBINANT DNA
TECHNOLOGY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
All questions carry equal marks.
1. Trizol.
2. Protinase K.
3. Restriction enzyme.
4. Name the vectors for the construction of genomic DNA
library.
5. Strategy of T/A cloning.
6. Hotstart PCR.
7. Application of Reverse Transcriptase PCR.
8. Expression vector.
9. Difference between SDS-PAGE and Native PAGE.
10. What is Zymogram?
Sub. Code
501304
RW-840
2
Ws9
Part B x 5 25)
Answer all questions, choosing either or
11. Describe the principle of DNA quantification by
Di-phenyl amine method.
Or
Give a detail account on Agarose gel
Electrophoresis.
12. Explain the concept of genomic DNA library
preparation.
Or
Give a short note on M13 vector with a neat sketch.
13. Elaborate in detail DGGE.
Or
Give a detail account on DNA microarray.
14. Write a significance of antimicrobial genes in gene
expression study.
Or
How do you study the expression of GFP tagged
proteins in C. elegans model system?
15. Describe the principle of TLC.
Or
Describe the principle of Western Blotting.
RW-840
3
Ws9
Part C x 10 30)
Answer any three questions.
16. Write briefly the principle of total RNA extraction from
the animal tissue.
17. Define hybridization. Give a detail account on Southern
hybridization.
18. Write a detail note on DNA labelling techniques.
19. Write a detail note on principle and applications of real
time PCR.
20. Write briefly the principle behind HPLC instrumentation
and its applications.
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