M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
First Semester
Biotechnology
Major Elective I BIOPHYSICS AND
BIOINSTRUMENTATION
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all the questions.
All questions carry equal marks.
1. Hydrophobic interactions.
2. pH.
3. -helix.
4. micro-RNA.
5. Dosimeter.
6. Dialysis.
7. Gel filtration.
8. Nano LC.
9. Fluorescence.
10. Electron microscopy.
Sub. Code
501501
RW-836
2
Sp 5
Part B x 5 25)
Answer all the questions, choosing either or
11. Explain ionic and peptide bonds with example.
Or
Describe about structure and properties of water.
12. Give a detailed account on structural
polymorphisms of DNA.
Or
What are the forces stabilizing the structure of
protein?
13. Describe about the applications of radioactive
isotopes in biological studies.
Or
How will you measure the radio activity in
biological sample using GM counter?
14. Write a short notes on principle and applications of
TLC.
Or
Write short note on Capillary electrophoresis.
15. Write short notes on IR spectroscopy.
Or
Explain about the principle of fluorescence
microscopy.
RW-836
3
Sp 5
Part C x 10 30)
Answer any three questions.
16. Explain the principles of thermodynamics with their
importance.
17. Give a detailed account on the Ramachandran plot.
18. Explain in detail about osmosis and reverse osmosis.
19. Describe the principle and applications of Agarose Gel
Electrophoresis.
20. Give a short notes on principle and application of NMR.
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