M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
First Semester
Biotechnology
Core 6 LAB II — MICROBIOLOGY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
All questions carry equal marks.
Comments on
1. Bright field microscope.
2. Autoclave.
3. Negative staining.
4. Lyophilization.
5. Bacterial generation time.
6. Drug resistance microbes.
7. Probiotics.
8. Anaerobic chamber.
9. Cellulose test.
10. 165 rRNA sequencing.
Sub. Code
501106
RW-835
2
Wk6
Part B 5 25)
Answer all questions, choosing either or
11. Explain the working principle of phase contrast
microscope with diagram.
Or
Explain the working principle of fluorescence
microscope with diagram.
12. Explain the Gram staining procedure and the
principle behind that.
Or
Mention various techniques used for obtaining pure
bacterial culture.
13. Explain about the bacteria growth curve.
Or
Give an account on antibiotics assay.
14. Comments on microbial analysis in food samples.
Or
Account on different types of anaerobic culture
media.
15. Write a comment on culture independent bacterial
cultivation.
Or
Give the principle behind IMVIC tests.
RW-835
3
Wk6
Part C x 10 30)
Answer any three questions.
Draw diagram and flow chart wherever necessary.
16. Explain in detail about the sterilization and disinfection
methods in microbiology.
17. Describe in detail about preservation and maintenance of
microbial cultures.
18. Give a detailed note on factors affecting bacterial growth.
19. Write in detail about the mass cultivation of
commercially important microorganisms.
20. Give account on various biochemical test use for the
bacterial characterization.
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