M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
First Semester
Biotechnology
Core V — Lab I ANALYTICAL BIOCHEMISTRY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Section A (10 x 2 20)
Answer all the questions.
All questions carry equal marks.
1. Define Spectroflorimetry.
2. Explain Mass Spectroscopy.
3. Define Cytosol.
4. Give abbreviation of ALP.
5. What is Titrations?
6. Define Chromatography.
7. Define Ultrafilteration.
8. Define Rotors.
9. What is Radioactivity?
10. Define variance.
Sub. Code
501105
RW-834
2
Sp 1
Section B x 5 25)
Answer all the questions, choosing either or
11. Write a brief note on beers law.
Or
Give application of Atomic Absorption Spectroscopy.
12. Give short note on SGOT and SGPT.
Or
Write short notes on Estimation of blood glucose.
13. Give short note account on Buffers.
Or
Give short notes on Determination of PI value of
amino acids.
14. Write about the Ultrafilteration.
Or
Give short note on Gel permeation
Chromatography.
15. Write Difference between parametric and
non- parametric statistics?
Or
Give short note on Regression and Correlation.
RW-834
3
Sp 1
Section C x 10 30)
Answer any three questions.
16. Give brief sketch about determination of carbohydrate by
DNSA method and Protein estimation by Lowry's
method.
17. Give elaborate note on evaluation of risk of coronary
heart disease estimation of serum cholesterol.
18. Describe and Illustrate on separation of amino acids and
sugars by — paper chromatography and plant pigments
by TLC and HPTLC.
19. Describe the principle, instrumentation and application
of GC, FPLC and affinity chromatography.
20. Give detail account on analysis of variance ;x2 test; basic
introduction to Multivariate Statistics.
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