M.Sc. DEGREE EXAMINATION, NOVEMBER 2017.
Third Semester
Bioinformatics
PRINCIPLES OF GENE MANIPULATION
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
All questions carry equal marks.
1. What is a restriction enzyme?
2. Define the term cosmid.
3. What is function of Tag DNA polymerase?
4. What is a transgenic organism?
5. What is yeast artificial chromosome?
6. What is DNA library?
7. Define the term genetic transformation.
8. What is a gene therapy?
9. What is in vitro amplification?
10. Salient features of recombinant vaccine.
Sub. Code
502301
RW-849
2
Ws9
Part B x 5 25)
Answer all questions.
11. Explain in detail the structure of a plasmid.
Or
Explain with suitable illustration the process of
genetic manipulation.
12. Explain the process of DNA ligation.
Or
Explain in detail pyrosequencing.
13. Explain in detail the issues regarding recombinant
therapeutics.
Or
What are the forensic applications of rDNA
technology?
14. Explain the structure of a CRISPER-CAS system.
Or
Elaborate in detail application of CRISPER
technology in bacterial identification.
15. Elaborate in detail application of gene transfer
technology in agriculture.
Or
Explain in detail of biosafty of transgenic animals.
RW-849
3
Ws9
Part C x 10 30)
Answer any three questions.
16. Explain in detail the steps involved in any one method of
DNA sequencing.
17. Explain in detail the different stages of cloning.
18. Explain in detail the process of site directed mutagenesis.
19. Explain in detail the process of CRISPER-Case
technology.
20. Explain with neat illustrations the production of human
recombinant insulin.
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