DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, DECEMBER 2017.
BIO-TECHNIQUES IN BOTANY
(2008 onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
All questions carry equal marks.
x 20 100 marks)
1. Comment on isoelectric focusing.
Mention any five buffer salts and their applications.

Describe the principle and application of SDS-PAGE

2. Explain the role of electrophoresis in protein
analysis.
Write an account on the working of HPLC.
Explain the principle of confocal microscopy.
3. Explain the formula for sedimentation coefficient.

Describe the principles and functions of phase
contrast microscope.
Write short notes on MS.
Sub. Code
23
DE-2976
2
sp4
4. Explain the different types of rays emitted by
isotopes and their properties.
Discuss the role of radioactive isotopes in biology.
Write short notes on autoradiography.
5. Write short notes on DNA fingerprinting.
Explain the principles and applications of PCR.
Discuss the principles and applications of DNA
sequencing.
6. Describe Southern blotting techniques.
Comment on AFLP.
State the applications of DNA microarray.
7. Write about the various types of bioinformative
tools for analysis of plant genome.
Discuss the relationship between genome,
transcriptome and proteomes of plants.
Discuss about RAPD.
8. Explain the principles of MALDI-TOF.
Discuss the tracking gene expression in plant cells.

Comment on DNA hybridization.