M.Sc. DEGREE EXAMINATION, APRIL 2017.
Second Semester
Bioinformatics
Lab II BIOCHEMICAL AND MOLECULAR BIOLOGY
METHODS
(2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
All questions carry equal marks.
1. Draw any two biosafety symbols.
2. What is pH?
3. What is genonmic DNA?
4. What is the difference between Native-PAGE and SDS-
PAGE?
5. Abbreviate any four molecular markers.
6. What is the difference between primer and probe?
7. Define the principle of chromatography.
8. Write any four applications of chromatography.
9. List the basic parts of microscope.
10. What are the applications of ELISA?
Sub. Code
502204
RW-191
2
Ws19
Part B x 5 25)
Answer ALL questions.
11. Explain the principle of spectrophotometer.
Or
Discuss about protein quantification by Bradford's
method.
12. Explain how to isolate genomic DNA from human.
Or
Write the role of every reagent used in SDS_PAGE.
13. Explain the three basic steps in PCR.
Or
Draw a neat diagram representing RAPD
and AFLP.
14. Explain the working principle of Ion exchange
chromatography
Or
Explain the working principle of Thin layer
chromatogrphy.
15. Discuss briefly about live cell imaging microscopy.
Or
Write about antigen and antibody preparation.
RW-191
3
Ws19
Part C 10 30)
Answer any three questions.
16. Discuss in detail about the various biosafety rules and
regulations.
17. Explain the method of plasmid isolation from bacteria.
18. Explain in detail about the basic blotting techniques.
19. Describe the detailed working principle of HPLC and
FPLC.
20. Discuss about various immunoprecipitation techniques.
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