M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Genetics
GENETIC ENGINERRING
Day Date: Saturday, 18-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Section-I compulsory.
Attempt any four from Section-II.
Draw neat and labeled diagrams.
Section-I
Q.1 Rewrite the sentence after choosing the correct answer from the
given alternatives.
07
1g) Which of the following DNA polymerase don't have to
exonuclease activity?
Klenow Kornberg
Klenew Korenberg
2 is the technique used for characterizing large region of
chromosome.
RAPD Chromosome walking
PCR RFLP
3 In Maxam's Gilbert's method guanine is methylated by
Dumethyl Sulphonate Dinethy Succinate
Dimethyl Sulphate Dimethyl Acetate
4 Blue white selection method is an example of type of
screening method.
Direct Immunological
Hybridization Indirect
is a source of Ribonuclease A.
Bovine pancreas Aspergillus niger
Horse pancreas Pseudomonas Putida
6 Colony hybridization technique has been developed by
Murashigue Skoog M. Grustein D. S. Hogness
Mertz Davis Hugh Birnstil
vector is used in both prokaryotes eckaryotes.
M 13 Cosmid
Shuttle Phagemid
Definitions. 07
Genomic library
Microinjection
Endonucleases
Donar DNA
Exonucleases
Cosmid
Plasmid
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SLR-MK-432
Section II
Q.2 Explain chain termination method of DNA sequencing. 14
Q.3 Explain in detail RFLP add a note on its applications. 14
Q.4 Explain with example plasmid as a cloning vector. 14
Q.5 Answer any two from the following. 14
Explain how will you produce salt tolerant plant.
Describe direct DNA transfer method by using syringe needle.
Explain direct screening method for recombinants.
Q.6 Write short notes on. (Any two) 14
Explain the methods of labeling of probes.
Write a note on synthesis of human interferon.
Explain production of Hepatitis B recombinant vaccine.