M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Botany
Elective — PLANT TISSUE CULTURE
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. HEPA filter
2. Culture medium
3. Organ culture
4. Differentiation
5. Caulogenesis
6. Isopentenyl adenine
7. Antrogenesis
8. Heterokaryons
9. Secondary metabolites
10. Gene bank
Sub. Code
4MBO3E3
AFF-4910
2
Ws7
Part B x 5 25)
Answer all questions by choosing either or
11. Write about the basic layout of a tissue culture lab.
Or
What are the different modes of sterilization?
Explain in detail.
12. How will you prepare single cell culture by paper
raft nurse technique?
Or
Comment on petri dish plating technique for single
cell culture.
13. Describe the specific nutritsional requirements for
root and shoot differentiation.
Or
What are gametoclonal variations? Explain in brief
their advantages.
14. Describe the technique of anther and pollen culture
for getting haploid plants.
Or
Give an account of in vitro pollination and
fertilization.
15. Define secondary metabolites. Mention the types of
secondary metabolites found in plants.
Or
How the cells are immobilized for the production of
secondary metabolites?
AFF-4910
3
Ws7
Part C x 10 30)
Answer any three questions.
16. Explain the composition of some commonly used tissue
culture media.
17. Describe the different methods of cell suspension
cultures.
18. Explain somatic embryogenesis. Add note on the
application in plant tissue culture.
19. Write an essay on the isolation, culture and fusion of
protoplasts.
20. Discuss the application of plant tissue culture in forestry.
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