Zoology (Semester IV) (CBCS) Examination, 2017
ANIMAL BIOTECHNOLOGY
Day Date: Wednesday, 19-04-2017 Max. Marks: 70
Time: 2:30 PM to 05.00 PM
N.B.: Q.1 Q.2 and Q.6 are compulsory.
Attempt any two from Q.3, 4 and 5.
Q.1 Tick the correct alternative.(Per question 2 marks) 14
G
g
contamination in cell culture is difficult to control.
Fungal Bacterial
Mycoplasmal All of the above
2 Platelets are the cell fragments, lacking a nucleus, that breaks
off from a
Macrophage Megakaryocyte
Erythrocytes Monocyte
3 The lac operon encodes thee enzymes required for the
metabolism
Glucose Maltose
Fructose Lactose
4 cDNA can be formed from RNA by
transcriptase reverse transcriptase
fructose lactose
5 DNA only transposones possess genes which
Transposeose Reverse transcriptase
Transcriptase All the above
6 is a method used in molecular biology for detection
of specific DNA sequence in DNA samples.
Northern blotting Southern blotting
Western blotting All the above
7 Transposons also called as
Jumping genes Transposable elements
Mobile genetic elements All the above
Q.2 Describe in detail primary cultures and add a note on cell clones. 14
Q.3 Explain the principle and technique of nucleic acid hybridization. 07
Page 2 of 2
Explain the principle and working of PCR. 07
Q.4 Explain western blotting technique. 05
Explain Operon concept. 05
What is RNA processing? 04
Q.5 Give in detail mechanism of transposition. 07
Explain the regulation of gene expression in eukaryotes. 07
Q.6 Write note on (any four) 14
Ethical issues in human cloning and biotechnology.
Application of genetic engineering in agriculture.
Somatic hybridization
FISH
Cybrids
Heterochromatinization