B.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Fifth Semester
Microbiology and Clinical Lab Technology
Elective PCR BASED DIAGNOSTICS
(CBCS 2015 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Annealing
2. Primer Tm value
3. Chloroform
4. Template
5. Taq polymerase
6. DNA profiling
7. DMSO
8. Random primer
9. DNA Sequences
10. DNA labelling
Sub. Code
5BMC1E2
AFF-5013
2
Wk ser
Part B x 5 25)
Answer all the questions.
11. How will you choose the correct temperature for a
PCR reaction?
Or
Write the basic steps in PCR experiments.
12. How will you synthesis cDNA molecule?
Or
Write the principle and procedure for Northern
blotting technique.
13. What is a probe? How will you synthesis DNA
probe?
Or
Discuss about the microbial diagnosis technique
with special reference to PCR.
14. Describe the procedure and importance of RAPD.
Or
Write about the principle, procedure and application
of Real time PCR.
15. Comment on automated sequencing.
Or
Explain about the DNA sequencing by Maxam
Gilbert method.
AFF-5013
3
Wk ser
Part C x 10 30)
Answer any three questions.
16. Write in detail the history, principle and reaction cycle of
PCR technique.
17. What is a plasmid? Write the procedure for the isolation
and identification of plasmid.
18. Discuss the role of radioactive and non-radio active
probes in genomic studies.
19. Describe in detail the procedure and application of DNA
finger printing technique.
20. What are the methods of annotation of genomes
sequences?
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