M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Genetics
ANALYTICAL INSTRUMENTS AND TECHNIQUES
Time: 2½ Hours Max. Marks: 70
Instructions: Part-I, question 1 is compulsory.
Attempt any four questions from Part II
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
All questions carry equal marks.
PART I
Q.1 Rewrite the sentence after choosing correct answer from the given
alternatives:
07
is the process of enlarging something only in
appearance not in physical size.
Magnification Resolving power
Focal length Numerical aperture
invented phase contrast microscope.
Galileo Frederick Zernik
Sanger E. Ruska
A biologically significant radioactive isotope is
14N 18O
14C 11C
Ratio of determines the stability of isotopein nature.
Electron to Proton Proton to Neutron
Electron to Neutron Neutron to Proton
The purpose of using stacking gel is to proteins.
Concentrate Analyse
Distribute Separate
is extensively used chromatographic technique to
determine base composition of nucleic acid.
Affinity Ion exchange
Adsorption GLC
The region below 2000Ao is known as
Visible region Infrared region
Vacuum ultraviolet region Near ultraviolet region
Q.1 Define the following terms. 07
Microscope
Electrophoresis
Radioactivity
Chromatography
Colorimeter
Mobile phase
Lens
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SLR-VK-321
PART II
Answer any four of the following:
Q.2 Discuss in detail optical principles of microscopy. 14
Q.3 Describe in detail colorimetry. 14
Q.4 Explain discontinuous gel electrophoresis. 14
Q.5 Answer any Two of the following:
Derive an equation for basic principles of electrophoresis.
Explain in short Ascending paper chromatography.
Write a note on types of radioactive decay.
14
Q.6 Answer any Two of the following: 14
Write a note on solid scintillation counter.
Discuss Bright field microscopy.
Write a note on gel permeation chromatography.