M.Sc. (Semester II) (CBCS) Examination Nov/Dec-2018
Genetics
ADVANCED MICROBIAL GENETICS
Time: 2½ Hours Max. Marks: 70
Instructions: Section I is compulsory.
Answer any four questions from Section II.
Section I
Q.1 Rewrite the sentence after choosing correct answer from the given
alternatives:
07
is used for production of citric acid .
Lactococcus lactic Aspergillus niger
Srepttococcus mutans Both a and b
The process of self-fertilization in fungi is known as
automixis amphimixis
spermatization somatogamy
The bacterial strain requiring amino acid supplements for growth is
termed as
Oligotroph Auxotroph
Prototroph Autotroph
Riboflavin is produced industrially by
Ashbya gossypii Fusarium venenatum
Monoascus purpureus Lactococcus lactic
method is used for isolation of drug resistant mutants.
Plaque counting Colony isolation
Replica plating Induction
When Hfr strains of E.Coli are crossed with F− strains, recombinants
obtained are
Always F Always Hfr
Rarely F Rarely HFr
cells are formed by
Integration of F factor into the chromosome
Entry of F factor into the cell
Excision the F factor from the chromosome
None of these
Q.1 Define the following terms. 07
Competency
Plasmid
Generalized transduction
Temporal mapping
Interrupted matting
Electroporation
Ori C
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SLR-VK-314
Section II
Q.2 Discuss bacterial recombination process which requires direct contact of two
cells.
14
Q.3 What is temperate phage? Describe mechanism of specialized transduction. 14
Q.4 Describe the bacterial recombination by uptake of free or naked DNA 14
Q.5 Answer any two of the following:-
Write in brief on evidence of spontaneous nature of mutation.
Describe analysis of mutation in biochemical pathways.
Brief the methods of isolation of phage resistant mutants.
14
Q.6 Write short notes on any two of the following: 14
Fungi in biotechnology
Yeast matting type switching mechanism
Fungal life cycle