M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Nano-Technology
ANIMAL BIOTECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section-I, question 1 is compulsory. Attempt any four questions from Section II All questions carry equal marks. Draw neat and labeled diagrams wherever necessary.
Section I
Q.1

Rewrite the sentence after choosing correct answer from the given alternatives:
07
Diffusion of nutrients and gases is often limited to explants over the size of
200
250
20
100
Loeb first attempt in 1897.
Cell culture
Transformation
Organ culture
All of these
Organ culture can be performed using
Raft method
Plasma clot method
Grid method
All of these
Recombinant proteins are
Proteins synthesized in animals
Proteins synthesized by transgene in host cells by rDNA technology.
Proteins synthesized in cells that are produced by protoplast fusion
Proteins synthesized in mutated cells
The production of complete animals from somatic cells of an animal is called
Cell cloning
Animal cloning
Gene cloning
All of these
The first successfully cloned animal was
Rabbit
Sheep
Rat
Dog
The method in which the nucleus of a donor cell is relocated to an enucleated target cell is known as
Cell transformation
Nuclear transplantation
Organ transplant
All of these
Page 2 of 2
SLR-UV-662
Q.1

Define the following terms.
07
Contact inhibition
Anchorage dependent tissue culture system
Primary cell culture
Passaging
Finite cell lines
Cryopreservation
CO2 incubator
Section II
Answer any four of the following:
Q.2
Classify and explain in details the different levels of bio-safety.
14
Q.3
Write in detail different types of tissue culture with diagrams.
14
Q.4
Explain vaccine production using cell culture in detail.
14
Q.5
Explain in detail about transgenic sheep.
14
Q.6
Answer any Two of the following:
14
Explain MTT Assay with suitable diagram.
Describe different types centrifugation with their principle.
Describe method of cell synchronization.
Q.7
Write short notes on. (Any Two)
14
Describe principle of flow cytometry and write its applications.
Write short note on Serum free media.
Short note on chemical sterilization.