B.Pharmacy (Semester III) (New CBCS) Examination, 2018
pharmaceutical Microbiology
Day and Date Thursday, 13-12-2018 Max. Marks 75
Time 10.30 a.m. to 1.30 p.m.
1. Multiple Choice Questions (20×1=20)
The purpose of staining is to demonstrate
Cell size Cell shape
Arrangement of bacterial cell All of above
The process of autoclaving is
Only vegetative cell gets killed
Spores and vegetative cell gets killed
Spores get killed only
Nucleus of the cell gets killed
Efficiency of HEPA filter is
99.97 88.87 90.97 98.97
The media in which nutritional environment is provided in such a way that
growth of certain bacteria is enhanced than other is called
sugar media transport media
enriched media enrichment media
Radiation used for cold sterilization are
X-ray Gamma rays Cosmic rays All of above
The phase of maximum multiplication result in increased population of
bacteria with reduced generation time is
log phase lag phase
decline phase stationary phase
Testing which confirms that products are free from the presence of viable
microorganisms is known as
sterility testing pyrogen testing
minimum inhibitory coefficient none of above
A fully assembled infectious virus is called as
micron prion virion none of above
Seat
No. Set P
Set P
SLR-TV 17 *SLRTV17*
The mycellum is complex of several filaments called
Lumen Hyphae Septum Plasmalemma
10) DNA present in bacteria is
Single, circular Single, linear
Single, crossed Double, circular
11) Which of the following methods are used for obtaining pure culture of
microorganisms
Streak plate method Pour plate method
Spread plate method All of above
12) The stain which differentiates two groups of bacteria in a mixture is known as
Negative stain Simple stain
Differential stain Indirect stain
13) Which of the following are chemical indicators of sterilization
Browne's tube Witness tube
Royce Sachet All of above
14) Test based on the rise of body temperature of rabbit is
Sterility testing Pyrogen testing
Minimum inhibitory coefficient None of above
15) Best autoclaving is achieved at
121°C for 15 min. 120°C for 1 hr.
110°C for 2 hrs. 100°C for 3 hrs.
16) Microorganisms which grow in absence of oxygen are called
Aerobic Microscopic Anaerobic None of above
17) Dop test is used for validation of
Membrane filter HEPA filter
Aseptic room Autoclave
18) Citrate utilization test is usually performed on
Chocolate agar Simmon's citrate agar
Nutrient agar MacConkey agar
19) On the outer surface of the viral envelope, glycoprotein subunits exposed
in the form of projecting spikes are known as
Capsid Capsule Peplomers All of above
20) When finite cell line undergoes transformation and acquires the ability to
divide indefinitely, it becomes
Continues cell line Discontinuous cell line
Infinite cell line Both b and c
Set P
*SLRTV17* SLR-TV 17
2. Answer any 2 of the following (2×10=20)
What is sterilization Classify and explain.
Write a note on designing of aseptic area.
Describe bacterial anatomy.
3. Answer any 7 of the following
Classify virus.
Write about assessment of microbial contamination and spoilage.
Describe about bacterial growth curve.
Explain about different culture media.
Describe about cultivation of viruses in chick embryo.
Describe cup plate method.
Write characteristic of ideal disinfectant.
Explain working and principle of autoclave.
What is IMViC test