M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Zoology
ANIMAL BIOTECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Q.1, 2 6 are compulsory.
Answer any two questions from Q.3, 4 and 5.
Q.1 Choose the correct alternatives and rewrite the sentence:- 14
is the rapid method of DNA cloning without host cell.
PCR FISH
GISH Blotting
mRNA of prokaryotes is
Monocistronic Polycistronic
Dicistromic Non cistronic
Lactose metabolism in E.coli is regulated by a system.
Repressible Co-repressible
Inducible Non-inducible
AUG is initiation codon codes for amino acid.
Arginine Methionine
Histidine Tryptophan
Protein sequencing was discovered by
Edman Gilbert
Koch Pasture
Nucleic acid will show maximum absorbance spectra at nm
290 270
280 260
is the termination codon.
UAA UAG
UGA All of the above
Q.2 What is gene expression? Explain in detail the regulation of gene expression in
Prokaryotes.
14
Q.3 Answer the following:-
Polymerase chain reaction 07
Splicing mechanism in mRNA 07
Q.4 Explain the following:-
Fluorescence in situ hybridization. 05
Application of genetic engineering in agriculture 05
Properties of stem cell 04
Q.5 Explain in short 07
Gene transfer methods 07
Protoplast fusion
Page 2 of 2
SLR-VS-514
Q.6 Write short notes (any 14
Primary cell culture
Sothern blotting
GISH
Edman degradation
Heterochromatin
Polyadenylation