M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Biotechnology
PLANT BIOTECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Choose the correct option and rewrite the sentence:- 14
Growth hormone producing apical dominance is
Auxin Gibberellin
Ethylene Cytokinin
The phenomenon of the reversion of mature cells to the meristematic state
leading to the formation of callus is known as
Redifferentiation Dedifferentiation
Cytodifferentiation Reformation
Somaclonal variations are the ones
Caused by mutagens Produce during tissue culture
Caused by gamma rays Induced during sexual embryogeny
Biolistic method is suitable for
Constructing recombinant DNA by joining vectors
Transformation of Plant cells
Disarming pathogen vectors
DNA fingerprinting
Hormone pair required for a callus to differentiate are
Auxin and cytokinin Auxin and ethylene
Auxin and absiccic acid Cytokinins and gibberllin
A medium which is composed of chemically defined compound is called
Natural media Artificial medium
Synthetic media Supernatural medium
The culturing of cells in liquid agitated medium is called
Liquid culture Micropropagation
Agar medium Suspension culture
Immobilized cell bioreactors are based on
Cell cultures on solid medium Cell cultures on liquid medium
Cell entrapped in gels Culture entrapped in cell
Artificial seeds are
Seeds produced in laboratory condition
Seeds encapsulated in a gel
Somatic embryos encapsulated in gels
Zygotic embryos encapsulated in gels
10) Hairy root cultures for secondary metabolite production are induced by
transforming plant cells with
Viruses Agrobacterium tumefaciens
Bacillus thuringiensis Agrobacterium rhizogenes
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SLR-VE-81
11) growth regulator responsible for fruit ripening.
Abscisic acid Ethylene
Gibberellic acid Auxin
12) Golden rice is rich in
Vitamin E Hormones
Provitamin A Growth hormones
13) Polyploidy is induced by
Irradiation Mutagenic chemicals
Ethylene Colchicine
14) Polyethylene glycol is
Fusogenic chemical Electrofusion stimulant
Callus stimulant Differentiation stimulation
Q.2 Answer the following any four:-
Plant tissue culture.
The concept of cybrid.
Callus culture
Molecular Farming
Symmetric and asymmetric hybrids
08
Write note on any two
Write a note on Artificial seeds Production.
Explain Industrial and therapeutic enzyme production using plant
biotechnology.
Write a note on haploid plant production.
06
Q.3 Answer the following. (Any Two)
Write the essential micro and macromolecules for plant nutrition.
Explain organogenesis.
Explain cell suspension culture.
08
Answer the following any
Write a note on Somaclonal variation.
Write a note on Somatic hybridization
06
Q.4 Answer the following. (Any Two)
Concept of organ culture.
What are viral vectors and explain the use of virulence DNA in gene
transfer mechanism.
Explain micro propagation.
10
Answer the following any
Describe gene silencing mechanism.
Give detailed account on tissue culture media.
04
Q.5 Answer the following. (Any Two) 14
Write in detail 'Cryopreservation technology'.
Explain various methods of Gene transfer in Plant biotechnology.
Explain Protoplast culture in detail.