DISTANCE EDUCATION
M.Sc.(Botany) DEGREE EXAMINATION, MAY 2018.
Specialization-Plant Bio-Technology
PLANT TISSUE CULTURE
(2008 onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each questions carries 20 marks.
x 20 100)
1. Define suspension culture. Give a general account of
growth patterns of cells in suspension culture.
Define nutrient medium. How to prepare a suitable
medium for callus induction in plants like tobacco?

Write note on surface sterilization of explants.
2. Write an essay on commercial importance and
applications of micropropagation.
Write notes on developing virus-free plants through
micropropagation.
Write notes on Thermotherapy and cryotherapy.
3. Write an essay on role of hormones in
organogenesis.
Write an essay on synthetic seed production.
Write about the applications of somoclonal variation

Sub. Code
14
DE-4078
2
Ws19
4. What is Shoot tip culture? Explain the principle and
advantages of Shoot tip culture.
Write notes on root culture and its applications.
Illustrate haploid culture from pollen.
5. Write an essay on various methods employed in
protoplast fusion technology.
Illustrate in vitro production of secondary
metabolites from plant cells.
Discuss on various factors influencing isolation of
protoplast.
6. Discuss the principle of somatic embryogenesis with
suitable examples.
Comment on 'cellular totipotency may be exploited
in plant tissue culture practices'
Write notes on embryo rescue.
7. Describe the principle and method of protoplast
isolation through mechanical and enzymatic
isolation methods.
Describe the principle of flower bud and ovule
culture.
Differentiate induced mutation and somoclonal
variations.
8. Write an essay on importance and applications of
protoplast fusion technology.
Write an essay on various sterilization methods
employed in preparing culture media and culture
room for plant tissue culture practices.
Write notes on basic components of tissue culture
media and their role in vitro regeneration of plant
cells.