Fill up the blanks appropriately:

PAS stains

Amyloid substances are better stain with and to be viewed under Polarised microscopy.

Perl's Prussian method is for staining

Oil red O stains all groups of in the tissues.

Calcium is stained by method.

Attempt all questions:

Harris Weigert method stains for calcium salts in tissues.

Verhoeff's method stains elastic fibres.

Pre analytical check up of quality control in histopathology is mandatory in SOP of quality assessment.

Mixture of Sudan III and Sudan IV is commonly used for fat stain.

Restoration of specimen for museum preservation, Kesserling solutions are considered essential for quality maintenance.

2. Write in brief on any two of the following:

Barr body

Immuno histochemistry

Tissue processing for frozen sections

3. Write short notes on any four of the following:

FNAC Preparation and Procedures

Masson's Trichrane s.tain

Amyloid stains

Frozen sections and automation

Embedding

4. Answer any three of the following:

Enumerate connective tissue stains. Write principle and procedure of Vongie Son Stain method.

Define cytogenetics. Explain the terms with illustration.

Replication

Transcription

Translation

Write in brief organisation of histopathology department and enumerate pre-analytical procedures of tissue processing.

What is Karyo typing? Give its procedure for preparation of materials. Enumerate clinical significance of Karyo typing.